Contribution of haematological factors to the distributions and estimation of Eustrongyliides africanus larvae densities in Clarias gariepinus and Clarias anguillaris from Bida floodplain of Nigeria

The contributions of hematological factors to the distribution and estimations of Eustrongylides africanus larvae densities in Clarias gariepinus and C. anguillaris of Bida floodplain of Nigeria were documented for the first time. The hematological factors making the most important contributions to the distributions of E. africanus larvae infections in clarias species are mean corpuscular haemoglobin concentration (MCHC), mean corpuscular haemoglobin (MCH), mean corpuscular volume (MCV) and neutrophils count, in descending order of magnitude; having the manifestations for the months of January, March, September, and December of the year being closely related. Five haematological factors (neutrophils, lymphocytes and eosinophils counts; MCH and MCV) having positive or negative correlation coefficient (r) between 0.50 and 0.85 contributed to the estimated of E.africanus larvae densities in the wild population of Clarias species

Cell types in the peripheral blood of waking catfish Clarias batrachus (Lin.)

Different types of haematocytes found in the peripheral blood of walking catfish Clarias batrachus, have been characterized and identified using morphological, morphometric and cytochemical techniques. These cells are: erythrocytes, reticulocytes, large and small lymphocytes, thrombocytes, monocytes and polymorphonuclear leucocytes (neutrophils).

Gross clinical signs and haematological changes associated with artifical infection of Edwardsiella tarda in Koi Carp

The occurrence of diseases is a significant setback for successful aquafarming. One of the common fish bacterial disease syndromes, Edwardsiellosis is caused by Edwardsiella tarda, a gram-negative, rod shaped bacterium associated with several diseases of marine and fresh water fish. In this study, an attempt was made to observe and analyze the onset of clinical symptoms and certain haematological parameters in Koi Carp, Cyprinus carpio L., following artificial infection with Edwardsiella tarda. The disease progress was observed and the clinical symptoms were monitored over a period of 15 days following infection. Fish were sampled at three day intervals to analyse the haematological parameters: total erythrocyte counts (RBC), total leucocyte counts (WBC), haemoglobin content and differential leucocyte count. Clinical symptoms observed included: erratic swimming behaviour, loss of appetite, haemorrhages, dropsy and exophthalmia. There was a significant decrease in the total RBC and haemoglobin levels by the 3rd and 6th day post infection, and an increase thereafter. WBC counts were higher in all infected groups in comparison to the control group. A significant increase in the number of neutrophils was found in the infected group up to the 9th day and a decrease thereafter. The lymphocyte number was significantly less up to the 12th day while the monocyte counts were significantly higher up to the 12th day post infection. The results showed that the bacterium, E. tarda, is pathogenic to Koi Carp. The hematological changes and clinical signs in infected fish reported in this paper will be helpful in the identification and the control of this infection.

A study on cytochemical properties of white blood cells from Acipenseridae of southern Caspian Sea

The objective of this study was to study leukocytes characteristics of Acipenseridae of southern Caspian Sea stained by cytochemical stains in order to classify these cells more accurately. The samples were taken from Sturgeon propagation centers and International Sturgeon Research Institute in the north part of Iran. 10 fish were selected randomly from each age group (fingerling, 1, 2, 3 to 11 years) and blood samples were taken from caudal vein using syringes with no anticoagulant. Blood smears were prepared immediately and fixed by methanol. The smears then stained using cytochemical staining according to the Sigma-Aldrich instructions. Neutrophils were positive for Sudan Black-B(SBB), Periodic acid-Schiff (PAS) and Peroxidase (PER) but negative for choloroacetate esterase (CAE). Eosinophi is stained positively for PER and SBB and weakly for PAS, acid phosphatase (ACP), CAE and a-naphthyl acetate esterase (a-NAE). Basophil was absent in the studied blood smears. Lymphocytes were positive for ACP but weak for PAS, CAE and a-NAE. Monocytes stained positively for B-glucoronidase (BG) in Acipenser persicus 5 and 11 years group, in Acipenser stellatus 1 and 2 years group, in Acipenser gueldenstaedtii 1 year group, in Acipenser nudiventris 9 year and in Huso huso 7 year group and stained weakly for a-NAE.